<rp id="nbgog"><acronym id="nbgog"><input id="nbgog"></input></acronym></rp><em id="nbgog"></em>
<progress id="nbgog"></progress><progress id="nbgog"></progress>

  • <button id="nbgog"></button>
    <li id="nbgog"><acronym id="nbgog"></acronym></li>
    <dd id="nbgog"><track id="nbgog"></track></dd>
    1. 仔豬日糧添加蛋白酶對豆粕致敏蛋白降解和腸道健康的影響

      發布單位:天津瑞孚農牧科技集團有限公司

      查看次數:218

      時間:2020-12-08
      開展兩個試驗研究豆粕型基礎日糧中添加蛋白酶對豆粕致敏蛋白(大豆球蛋白和β-伴大豆球蛋白)降解和斷奶仔豬腸道健康的影響。

      試驗1中收集了兩個不同來源的兩個豆粕樣品,進行豬胃體外降解實驗,研究蛋白酶(15000 U/kg飼料原料)對豆粕致敏蛋白降解的影響。試驗二,選用48頭斷奶仔豬(體重6.66kg),每批24頭,共兩批。豬只單獨飼喂在代謝籠中,總共4種日糧(12頭/日糧)。日糧為玉米-豆粕型,試驗處理為2(豆粕1、豆粕2)×2(蛋白酶添加量為0、15000U/kg)雙因子試驗。日糧飼喂10天,然后屠宰,檢測小腸組織形態、小腸滲透性,以及血清促炎因子水平。

      豆粕1、豆粕2的粗蛋白含量分別為46.9%、47.7%,大豆球蛋白為14.0%和14.6%,β-伴大豆球蛋白為9.90%和10.3%。試驗中未發現蛋白酶和豆粕之間有互作。蛋白酶的添加有提高大豆球蛋白體外胃液降解的趨勢(P=0.069)。日糧中添加蛋白酶降低了空腸異硫氰酸熒光素葡聚糖 4000 Da(腸道通透性的標記探針,P=0.099),并顯著降低了血清TNF-α濃度(P=0.037)。蛋白酶對小腸組織形態沒有顯著影響。

      總之,蛋白酶有提高大豆球蛋白胃液降解的趨勢,并降低腸道通透性、血清促炎因子的濃度,結果表明斷奶仔豬的豆粕型日糧中可以添加蛋白酶改善腸道健康。


      Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets

      Two experiments were conducted to determine the effects of protease supplementation on degradation of soybean meal (SBM) allergenic proteins (glycinin and β-conglycinin) and gut health of weaned pigs fed soybean meal-based diets. In experiment 1, 2 SBM samples from 2 different sources were subjected to porcine in vitro gastric degradation to determine the effects of protease (at 15,000 U/kg of feedstuff) on degradation of the soybean allergenic proteins. In experiment 2, 48 weaned pigs (body weight = 6.66 kg) were obtained in 2 batches of 24 pigs each. Pigs were individually housed in metabolic crates and fed 4 diets (12 pigs/diet). The diets were corn-based diet with SBM 1 or SBM 2 without or with protease at 15,000 U/kg of diet in 2 × 2 factorial arrangement. Diets were fed for 10 d and pigs were sacrificed on day 10 for measurement of small intestinal histomorphology, permeability of small intestine mounted in Ussing chambers, and serum concentration of pro-inflammatory cytokines. Two SBM sources (SBM 1 and SBM 2) contained 46.9% or 47.7% CP, 14.0% or 14.6% glycinin, and 9.90% or 10.3% β-conglycinin, respectively. Protease and SBM source did not interact on any of the response criteria measured in the current study. Protease supplementation tended to increase (P = 0.069) the in vitro gastric degradation of glycinin. Protease supplementation tended to reduce (P = 0.099) fluorescein isothiocyanate dextran 4,000 Da (which is a marker probe for intestinal permeability) flow in jejunum, and reduced (P = 0.037) serum TNF-α concentration. Protease did not affect small intestinal histomorphology. In conclusion, protease tended to increase gastric degradation of glycinin and reduce gut permeability, and serum concentration of pro-inflammatory cytokines, indicating that the protease used in the current study can be added to SBM-based diets for weanling pigs to improve gut health.

      文章來源:豬營養國際論壇
      【免責聲明】:文章來源于網絡,我們對文中陳述觀點判斷保持中立,并不對文章觀點負責。僅供讀者參考。版權屬于原作者。
      色综合久久五月色婷婷-色综合久久中文综合网-色综合伊人色综合网站